Male genetic infertility problem: what to do? HRSS? Flow Cytometry?

[zoelaura]

Hello,

My partner is 35 and has a male genetic infertility problem: most of his sperm do not carry DNA. Some sperm carry half of DNA and very few sperm carry normal DNA. His sperm has normal morphology values but sperm count is low.
In 2003, with his ex-wife, they tried several insemination and in-vitro fertilization through ICSI (with testicular open biopsy and sperm hand-selected at about a magnification of 500x based upon Motility and Morphology). All trials were unsuccessful because no egg fertilized.
We want to know whether we should try:

HIGH-RESOLUTION SPERM SELECTION (HRSS)

the above will then be followed by intra-cytoplasmic sperm injection (ICSI).

In addition, we have one question: Is Flow Cytometry only used for differentiating chromosomes X and Y (because FISH uses DNA probes that specifically attach to either the X or Y chromosome in sperm) or can this technique be used in our case (to measure quantity of DNA present in the sperm)? Can Flow Cytometry be used to select sperm with normal DNA?

We will gett a sperm donor if needed. We just want to make sure before that we have realistically no chance to have a biological child together despite the recent advances of science. That's why I am searching around. My partner and his previous partner tried 7 years ago and I want to check whether there have been any advances in the last years that would make a difference for us.

Thank you

[foogball]

Hi zoelaura,

Welcome to Fertile Thoughts!

Here are a few links to forums here to post your question...so you get the best advice/help!

Donor Issues (Egg & Sperm) - Fertile Thoughts

General Infertility - Fertile Thoughts

Genetic Issues - Fertile Thoughts

IVF & High Tech - Fertile Thoughts

The general infertility is the largest forum here...many women are on there daily. I would try posting there, and even on a couple of the other forums above. That way you can get the most results (as far as support/advice, etc)

Again, welcome!

If you ever have any questions, let me know!
Molly