cassie....i think one of the more important things would be to try and keep your test time as consistent as possible. testing one day in the morning and the next day before bed leaves too much margin for missing your window. that's a 36 hour difference or more. during that time you could surge, O, and completely miss your opportunity.
if you only plan on testing once a day, then try to keep the testing at 24 hour intervals or less. if you have to pee before you leave work, then i'd do it then. OPK's are small enough that you can keep them in your pocketbook.
if you feel you wouldn't be able to be consistent with that time each day, then i'd probably do it at bedtime and use that as your test time each day.
so in answer to your question, in this case, consitency is very important.
keep in mind that a pos OPK is indicative of O'ing within 12-36 hours. if you can't keep your test time (once a day) at a max of 24 hour intervals, then i'd bite the bullet and test 2x a day so at least you know you can be a little inconsistent, KWIM?
good luck!